I am interested in uncovering novel mechanisms regulating white blood cell formation.  Using an unbiased approach our laboratory has identified a number of proteins required for myelopoiesis. We are interested in determining the target molecular networks of these proteins to identify previously unknown pathways controlling the development of white blood cells in health and disease. This provides potential for the design of innovative therapeutics.

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Keightley MC, S. Markmiller, C.G. Love, J.E.J. Rasko, G.J. Lieschke, J.K. Heath

Experimental approaches to studying the nature and impact of splicing variation in zebrafish

The Zebrafish: Genetics, Genomics, and Transcriptomics, 4th Edition, Elsevier 2016.

Keightley MC, Wang C-H, Pazhakh V, Lieschke GJ.

Delineating the roles of neutrophils and macrophages in zebrafish regeneration models.

Int J Biochem Cell Biol. 2014 Nov;56C:92-106

Markmiller S, Cloonan N, Lardelli R, Doggett K, Keightley MC, Boglev Yx, Trotter AJ, Ng AY, Verkade H, Wilkins SJ, Ober EA, Field HA, Grimmond SM, Lieschke GJ, Sainier DYR, Heath JK

Minor class splicing shapes the zebrafish transcriptome during development.

Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3062-7.

Keightley MC, Crowhurst MO, Layton JE, Beilharz T, Markmiller S, Varma S, Hogan BM, de Jong-Curtain TA, Heath JK, Lieschke GJ.

In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation.

FEBS Lett. 2013 Jul 11;587(14):2150-7. doi: 10.1016/j.febslet.2013.05.030. Epub 2013 May 25.

Keightley MC, Wong BBM, Lieschke GJ.

Immune priming: Mothering males modulate immunity.

Curr Biol. 2013 Jan 21;23(2):R76-8. doi: 10.1016/j.cub.2012.11.050.

Pase L, Layton JE, Wittmann C, Ellett F, Nowell CJ, Reyes-Aldasoro CC, Varma S, Rogers KL, Hall CJ, Keightley MC, Crosier PS, Grabher C, Heath JK, Renshaw SA, Lieschke GJ

Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish.

Curr Biol. 2012; 22(19):1818-1824

Keightley MC, Layton JL, Hayman JW, Heath JK, Lieschke GJ.

Mediator subunit 12 is required for neutrophil development in zebrafish.

PLoS One 2011 6(8): e23845