I am interested in uncovering novel mechanisms regulating white blood cell formation.  Using an unbiased approach our laboratory has identified a number of proteins required for myelopoiesis. We are interested in determining the target molecular networks of these proteins to identify previously unknown pathways controlling the development of white blood cells in health and disease. This provides potential for the design of innovative therapeutics.

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Keightley MC, Carradice DP, Layton JE, Pase L, Bertrand JY, Wittig JG, Dakic A, Badrock AP, Cole NJ, Traver D, Nutt SL, McCoey J, Buckle AM, Heath JK, Lieschke GJ.
The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger.

Nat Commun. 2017 Apr 6;8:14911. doi: 10.1038/ncomms14911.

Pazhakh V, Clark S, Keightley MC, Lieschke GJ.

A GCSFR/CSF3R zebrafish mutant models the persistent basal neutrophil deficiency of severe congenital neutropenia.

Sci Rep. 2017 Mar 10;7:44455. doi: 10.1038/srep44455.

Keightley MC, Nilsson SK, Lieschke GJ.

MED12 in hematopoietic stem cells – cell specific function despite ubiquitous expression.

Stem Cell Investig. 2017; 4: 3. Published online 2017 Jan 18. doi:  10.21037/sci.2016.12.04.

Keightley MC, S. Markmiller, C.G. Love, J.E.J. Rasko, G.J. Lieschke, J.K. Heath.

Experimental approaches to studying the nature and impact of splicing variation in zebrafish.

The Zebrafish: Genetics, Genomics, and Transcriptomics, 4th Edition, Elsevier 2016.

Keightley MC, Wang C-H, Pazhakh V, Lieschke GJ.

Delineating the roles of neutrophils and macrophages in zebrafish regeneration models.

Int J Biochem Cell Biol. 2014 Nov;56C:92-106.

Markmiller S, Cloonan N, Lardelli R, Doggett K, Keightley MC, Boglev Y, Trotter AJ, Ng AY, Verkade H, Wilkins SJ, Ober EA, Field HA, Grimmond SM, Lieschke GJ, Sainier DYR, Heath JK.

Minor class splicing shapes the zebrafish transcriptome during development.

Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3062-7.

Keightley MC, Crowhurst MO, Layton JE, Beilharz T, Markmiller S, Varma S, Hogan BM, de Jong-Curtain TA, Heath JK, Lieschke GJ.

In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation.

FEBS Lett. 2013 Jul 11;587(14):2150-7. doi: 10.1016/j.febslet.2013.05.030. Epub 2013 May 25.

Keightley MC, Wong BBM, Lieschke GJ.

Immune priming: Mothering males modulate immunity.

Curr Biol. 2013 Jan 21;23(2):R76-8. doi: 10.1016/j.cub.2012.11.050.

Pase L, Layton JE, Wittmann C, Ellett F, Nowell CJ, Reyes-Aldasoro CC, Varma S, Rogers KL, Hall CJ, Keightley MC, Crosier PS, Grabher C, Heath JK, Renshaw SA, Lieschke GJ.

Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish.

Curr Biol. 2012 Oct 9;22(19):1818-24. doi: 10.1016/j.cub.2012.07.060. Epub 2012 Aug 30.

Keightley MC, Layton JL, Hayman JW, Heath JK, Lieschke GJ.

Mediator subunit 12 is required for neutrophil development in zebrafish.

PLoS One. 2011;6(8):e23845. doi: 10.1371/journal.pone.0023845. Epub 2011 Aug 25.