Gene targeting is a genetic technique used to remove, add or change information in genes. It is also a method to introduce mutations.
This is usually done at a very specific time during an organism’s development or life to change its appearance, behaviour or biochemical characteristics. During genetic targeting, scientists remove (knock out) or add (knock in) genes to study the function of a particular gene or the effect of a particular mutation.
The most common application of gene targeting is to study human genetic diseases, but it is also used to study the mechanisms of gene expression and regulation. They key benefit of gene-targeting is that it provides information in a physiologically-relevant context, as the effects of the genetic changes are observed within the organism itself rather than on an isolated cell culture.
The Gene Targeting facility
The Monash Gene Targeting facility provides researchers with gene recombineering, embryonic stem (ES) cell and gene targeting services.
Our team has an established track record of delivering targeting and transgenic constructs for any animal model, targeted mouse/rat ES clones, and knock in/out transgenic mice or rats. This has garnered us a reputation which has seen our services requested both internationally and Australia-wide.
When projects are fully completed within our facility, we are able to guarantee successful mouse germ line transmission.
With all the services provided by Gene Targeting, we consult with researchers to ensure all requirements are met.